micropatterning of ecm proteins on glass substrates to regulate cell attachment and proliferation

Micropatterning of ECM Proteins on Glass Substrates to Regulate Cell Attachment and Proliferation

BACKGROUND Micropatterning is becoming a powerful tool for studying cells in vitro. This method not only uses very small amount of material but also mimic the microenvironment structure present in living tissues better than flask culturing techniques. In previous studies using micropatterning of extracellular matrix proteins on glass surfaces, the rate of protein detachment from the surface was...

Using self-assembled monolayers to pattern ECM proteins and cells on substrates.

We present a method that uses microcontact printing of alkanethiols on gold to generate patterned substrates presenting "islands" of extracellular matrix (ECM) surrounded by nonadhesive regions such that single cells attach and spread only on the adhesive regions. We have used this micropatterning technology to demonstrate that mammalian cells can be switched between growth and apoptosis progra...

Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner.

A novel strategy for micropatterning proteins on the surface of polyhydroxyalkanoate (PHA) biopolymer by microcontact printing (microCP) is described. The substrate binding domain (SBD) of the Pseudomonas stutzeri PHA depolymerase was used as a fusion partner for specifically immobilizing proteins on PHA substrate. Enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) fuse...

Effects of ECM Proteins and Cationic Polymers on the Adhesion and Proliferation of Rat Islet Cells

The effects of extracellular matrix (ECM) proteins and cationic polymers on the adhesion and proliferation of rat islet cells, RIN-5F cells, were investigated. ECM proteins of laminin, fibronectin, vitronectin, type I collagen, type II collagen, and type IV collagen, and cationic polyelectrolytes of poly(L-lysine) and poly(allylamine) were coated on the wells of polystyrene cell culture plates....

Micropatterning Co-cultures of Epithelial Cells on Filter Insert Substrates

A key feature of mature epithelium is the presence of an apical-basal polarization. In vitro this is achieved by culturing either pure populations of epithelial cells or unorganized mixtures of primary cell populations harvested from epithelial tissues in a filter insert culture system. In addition to epithelial cells however, multiple other cell types are also present in normal epithelium in a...

Micropatterning of cell attachment on pSi films by direct laser writing